Protein Variants | Comment | Organism |
---|---|---|
additional information | the enzyme from Serratia marcescens is overexpressed in Lactobacillus diolivorans to produce meso-2,3-butanediol (meso-2,3-BTD). A two-step cultivation process with Serratia marcescens is developed for production of 2-butanol in Lactobacillus diolivorans via vitamin B12-dependent diol dehydratase (PduCDE) and alcohol dehydrogenase (pduQ). In the first step of the process, Serratia marcescens is used to produce stereospecifically meso-2,3-BTD from glucose followed by heat inactivation of Serratia marcescens. The accumulated meso-2,3-BTD is then converted during anaerobic fermentation with glucose into 2-butanol by Lactobacillus diolivorans. The process yields a butanol concentration of 10 g/l relying on wild-type bacterial strains. A further improvement of the maximum butanol titer is achieved using an engineered Lactobacillus diolivorans strain overexpressing the endogenous alcohol dehydrogenase pduQ. The two-step cultivation process based on this engineered strain leads to a maximum 2-butanol titer of 13.4 g/l, which means an increase of 34% | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | Serratia marcescens | - |
(3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | Serratia marcescens DSMZ 14187 | - |
(3R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | H9XP47 | - |
- |
Serratia marcescens DSMZ 14187 | H9XP47 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | batch cultivations with glucose as sole carbon source are conducted. Acetate is co-utilised by Serratia marcescens as carbon source | Serratia marcescens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-butane-2,3-diol + NAD+ | - |
Serratia marcescens | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3S)-butane-2,3-diol + NAD+ | - |
Serratia marcescens DSMZ 14187 | (3R)-acetoin + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | UniProt | Serratia marcescens |
budC | - |
Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Serratia marcescens |
General Information | Comment | Organism |
---|---|---|
additional information | Serratia marcescens is a very efficient producer of meso-2,3-butanediol (meso-2,3-BTD)from glucose | Serratia marcescens |